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CONTENTS
1. General ................................................................................................................. 3
1.1 Instrument .................................................................................................................. 3
1.2 Reservations................................................................................................................ 3
1.3 Billing .......................................................................................................................... 3
1.4 Unauthorized use ........................................................................................................ 3
1.5 Users’ mailing list ........................................................................................................ 4
1.6 User guide ................................................................................................................... 4
1.7 Acknowledging MIU .................................................................................................... 4
1.8 Image processing and analysis ..................................................................................... 4
2. Microscope use ................................................................................................... 4
2.1 Settings for the light path ............................................................................................ 4
2.2 Objectives ................................................................................................................... 6
2.3 Fluorescence filters ..................................................................................................... 6
2.4 Transmitted (bright-field) light microscopy .................................................................. 7
2.4.1 Koehler illumination ............................................................................................. 7
2.4.2 Phase-contrast imaging ........................................................................................ 8
2.4.3 DIC imaging .......................................................................................................... 8
3. Imaging .............................................................................................................. 10
3.1 Zeiss AxioCam HRc 14-bit color CCD camera .............................................................. 10
3.2 Zeiss AxioVision software (version 3.1) ...................................................................... 10
3.2.1 Live view ............................................................................................................ 10
3.2.2 Adjustment of Camera Settings .......................................................................... 11
3.3 Display properties ..................................................................................................... 13
3.3.1 Adding a scale bar .............................................................................................. 14
4. Saving and transferring images ....................................................................... 15
4.1 File formats ............................................................................................................... 15
4.2 Back-up copies .......................................................................................................... 15
4.3 Saving images ............................................................................................................ 15
4.4 Saving images in Mcbserver1..................................................................................... 15
4.5 Saving images into your own computer ..................................................................... 16
4.6 Saving images into network folder............................................................................. 16
4.7 Saving images into a USB stick or CD ......................................................................... 16
4.8 Saving images locally ................................................................................................. 16
5. Ending your imaging session ........................................................................... 18
6. Troubleshooting ................................................................................................ 18
6.1 Microscope (both fluorescence and transmitted light imaging) ................................. 18
6.1.1 Fluorescence light microscopy ............................................................................ 18
6.1.2 Transmitted light microscopy ............................................................................. 19
6.2 Camera ..................................................................................................................... 19
6.4 AxioVision software ................................................................................................... 20
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