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Zeiss LSM 510 META manuali

Manuali dei proprietari e guide per l'utente per Microscopi Zeiss LSM 510 META.
We fornisce 6 manuali pdf Zeiss LSM 510 META per scaricare gratuitamente in base al tipo di documento: Manuale Utente, Specifiche


Zeiss LSM 510 META Manuale Utente (13 pagine)


Marchio: Zeiss | Categoria: Microscopi | Dimensione: 2.33 MB |

 

Indice

Start-up

1

Basic Image Acquisition

2

Halogen

5

Shut-down

12

Zeiss LSM 510 META Manuale Utente (66 pagine)


Marchio: Zeiss | Categoria: Microscopi | Dimensione: 3.95 MB |

 

Indice

Confocal microscopy

1

Confocal Principle

2

LSM 510 scan head

3

Axioplan 2 imaging

3

Axiovert 100M

4

LSM 510 META

5

Axiovert 200M

6

Turning on the lasers

12

Contents

15

Fluorescence is observed by

17

Choosing the configuration

20

Multi Track Configuration

23

Cy5-Cy3-FITC Multi Track

24

Adjusting the scan speed

27

Image Acquisition

30

Pixel sizeMagnification

31

Optical Zooming

32

1) Select Palette

33

2) Select Range Indicator

33

Red = Saturation (maximum)

33

Blue = Zero (minimum)

33

Saturation at the maximum

34

Gain set correctly

34

Amplifier Gain increases the

34

will need to be decreased

34

Photobleaching is linear!

35

Adjusting the Laser Intensity

36

Adjusting Gain and Offset

37

Line Averaging

40

Frame Averaging

41

Collecting an Averaged Image

42

Confocal Z Sectioning

46

Z - Series using Z Sectioning

48

Z Sectioning – Setting Range

49

Viewing a Z - Series

50

1) Select Gallery

51

2) Select Data for

51

Use Subset to

51

Multiple Regions of Interest

55

Time Series

56

Tiled Image

60

Saving Data - Using Database

62

Saving Data – Using Export

63

Shut Down Procedure

64

Please consult the manual

65

Zeiss LSM 510 META Manuale Utente (21 pagine)


Marchio: Zeiss | Categoria: Microscopi | Dimensione: 1.29 MB |

 

Indice

LSM 510 Meta

1

Training Notes

1

LSM 510 Meta Training Notes

10

Zeiss LSM 510 META Manuale Utente (56 pagine)


Marchio: Zeiss | Categoria: Microscopi | Dimensione: 3.96 MB |

 

Indice

Confocal microscopy

1

Turning on the lasers

7

(Main menu: Acquire)

10

Contents

13

Multi Track Configuration

17

Cy5-Cy3-FITC Multi Track

18

633 nm, using

18

1) Select Scan

19

3) Select the Frame Size

19

Pinhole size

23

= 1 Airy unit

23

Image Acquisition

24

Optical Zooming

26

1) Select Palette

27

2) Select Range Indicator

27

Red = Saturation (maximum)

27

Blue = Zero (minimum)

27

Photobleaching is linear!

29

Adjusting the Laser Intensity

30

Adjusting Gain and Offset

31

Line Averaging

34

Frame Averaging

35

Collecting an Averaged Image

36

Confocal Z Sectioning

40

Z - Series using Z Sectioning

42

Z Sectioning – Setting Range

43

Viewing a Z - Series

44

1) Select Gallery

45

2) Select Data for

45

Use Subset to

45

Multiple Regions of Interest

49

Time Series

50

1) If required, use

52

Saving Data - Using Database

54

Saving Data – Using Export

55

Zeiss LSM 510 META Specifiche (330 pagine)


Marchio: Zeiss | Categoria: Microscopi | Dimensione: 10.17 MB |

 

Indice

Laser Scanning Microscope

1

B 40-051 e 07/98 III

3

IV B 40-051 e 07/98

4

Scope of Equipment Supplied

6

INTRODUCTION

10

X B 40-051 e 07/98

10

CONTENTS

11

NOTES ON DEVICE SAFETY

12

Contents LSM 510

12

1-2 B 40-051 e 07/98

12

1 NOTES ON DEVICE SAFETY

13

Exit aperture

18

B 40-051 e 07/98 1-9

19

1-10 B 40-051 e 07/98

20

LGK 7774

21

LGK 7628-1

21

1-12 B 40-051 e 07/98

22

LSM 510 - SETUP REQUIREMENTS

24

2-2 B 40-051 e 07/98

24

Fig. 2-1

25

Fig. 2-2

25

Fig. 2-3

26

Fig. 2-4

27

2-12 B 40-051 e 07/98

34

CHAPTER 3 INTRODUCTION TO

35

LASER SCANNING MICROSCOPY

35

3-2 B 40-051 e 07/98

36

Module VIS

40

Scan ModuleMicroscope

40

Module UV

40

3-12 B 40-051 e 07/98

46

CHAPTER 4 OPERATION

59

4 OPERATION

61

Fig. 4-1

62

Fig. 4-2

63

Fig. 4-3

63

Fig. 4-4

64

Fig. 4-5

65

Fig. 4-6

66

Fig. 4-7

66

Fig. 4-8

67

Fig. 4-9

67

Fig. 4-10

68

Fig. 4-11

69

Fig. 4-12

70

Fig. 4-13

71

Fig. 4-14

72

Fig. 4-15

73

Fig. 4-16

74

Fig. 4-17

75

Fig. 4-18

76

Fig. 4-19

77

Fig. 4-20

78

Fig. 4-21

79

Fig. 4-22

80

Fig. 4-23

82

Fig. 4-24

82

Fig. 4-25

83

Fig. 4-26

83

Fig. 4-27

83

Fig. 4-28

84

Fig. 4-29

84

Fig. 4-30

85

Fig. 4-31

86

Fig. 4-32

88

Fig. 4-33

89

Fig. 4-34

90

Fig. 4-35

90

Fig. 4-36

91

Fig. 4-37

92

Fig. 4-38

93

Fig. 4-39

95

Fig. 4-40

96

Fig. 4-41

97

Fig. 4-42

98

Fig. 4-43

100

Fig. 4-44

101

Fig. 4-45

103

OPERATION

104

4-46 B 40-51 e 07/98

104

B 40-051 e 07/98 4-47

105

Fig. 4-48

106

Fig. 4-49

107

Fig. 4-50

108

Fig. 4-51

109

Fig. 4-52

110

Fig. 4-53

111

Fig. 4-54

112

Fig. 4-55

113

Fig. 4-56

114

Fig. 4-57

115

Fig. 4-58

115

Fig. 4-59

116

Fig. 4-60

117

Fig. 4-61

118

Fig. 4-62

119

Fig. 4-63

120

Fig. 4-64

121

Fig. 4-65

122

Fig. 4-66

123

B 40-051 e 07/98 4-67

125

Fig. 4-68

126

Colored marker positions

126

Fig. 4-69

127

Fig. 4-70

128

Fig. 4-71

129

Fig. 4-72

129

Fig. 4-73

130

Fig. 4-74

130

Fig. 4-75

131

Fig. 4-76

131

Fig. 4-77

132

Fig. 4-78

133

Fig. 4-79

133

Fig. 4-81

134

Fig. 4-80

134

Fig. 4-82

135

Fig. 4-83

135

Fig. 4-90

139

Fig. 4-91

140

LSM 510 Image Optimization

141

B 40-051 e 07/98 4-83

141

Fig. 4-93

142

Fig. 4-94

143

Fig. 4-95

144

B 40-051 e 07/98 4-87

145

Fig. 4-97

146

Fig. 4-98

147

Fig. 4-99

148

Fig. 4-100

149

Fig. 4-101

150

Fig. 4-102

151

Fig. 4-103

152

Fig. 4-104

153

/ Ampl. Gain

154

Fig. 4-105

155

Fig. 4-106

156

Fig. 4-107

157

Fig. 4-108

158

Fig. 4-109

159

Fig. 4-110

160

Fig. 4-111

161

Fig. 4-112

162

Fig. 4-113

163

Fig. 4-114

164

Fig. 4-115

164

Fig. 4-116

165

Fig. 4-117

166

Fig. 4-118

168

Fig. 4-119

169

Fig. 4-120

170

Fig. 4-121

171

Fig. 4-122

172

Fig. 4-123

173

Fig. 4-124

174

Fig. 4-125

175

Fig. 4-126

176

Fig. 4-127

177

Fig. 4-128

178

Fig. 4-129

179

Fig. 4-130

180

Fig. 4-131

181

4-124 B 40-051 e 07/98

182

Fig. 4-133

183

Fig. 4-134

185

Fig. 4-135

186

Fig. 4-136

187

Fig. 4-137

188

Fig. 4-138

188

Fig. 4-139

189

Fig. 4-140

190

Fig. 4-141

191

4-134 B 40-051 e 07/98

192

Fig. 4-143

193

Fig. 4-144

194

B 40-051 e 07/98 4-137

195

Fig. 4-146

196

Fig. 4-147

196

Fig. 4-148

198

Fig. 4-149

199

Fig. 4-150

199

Fig. 4-151

199

Fig. 4-152

200

Fig. 4-153

201

Fig. 4-154

202

Fig. 4-155

203

Fig. 4-156

203

Fig. 4-157

204

Fig. 4-158

205

Fig. 4-159

206

Fig. 4-160

206

Fig. 4-161

207

Fig. 4-162

207

Fig. 4-163

209

Fig. 4-164

210

Fig. 4-165

212

Fig. 4-166

214

Fig. 4-167

215

Fig. 4-168

216

Fig. 4-169

217

LSM 510 Annex

219

B 40-051 e 07/98 4-161

219

Annex LSM 510

220

4-162 B 40-051 e 07/98

220

B 40-051 e 07/98 4-163

221

4-164 B 40-051 e 07/98

222

B 40-051 e 07/98 4-165

223

4-166 B 40-051 e 07/98

224

B 40-051 e 07/98 4-167

225

4-168 B 40-051 e 07/98

226

B 40-051 e 07/98 4-169

227

4-170 B 40-051 e 07/98

228

Fig. 4-170 Filter change

229

Allen key

230

B 40-051 e 07/98 4-173

231

Fine focusing

233

Fig. 4-173

234

Fig. 4-174

237

Fig. 4-175

240

Fig. 4-176

246

Fig. 4-177

247

Fig. 4-178

249

Fig. 4-179

250

Fig. 4-180

252

Fig. 4-181

253

CHAPTER 5 3D FOR LSM 510

255

5-2 B 40-051 e 07/98

256

Positive rotation directions

257

Fig. 5-1

257

Fig. 5-2

259

Display window

260

Fig. 5-4

265

Fig. 5-5

267

Fig. 5-6

268

Fig. 5-7

270

Fig. 5-9

274

Fig. 5-10

275

Fig. 5-11

276

Fig. 5-12

278

Fig. 5-13

280

Fig. 5-14

283

Fig. 5-15

285

Fig. 5-16

287

Fig. 5-17

288

Fig. 5-18

291

Fig. 5-19

291

Fig. 5-20

292

Fig. 5-21

293

Fig. 5-22

295

Fig. 5-23

297

Fig. 5-24

299

Fig. 5-25

301

Fig. 5-26

303

Fig. 5-27

304

Fig. 5-28

305

Fig. 5-29

306

Fig. 5-30

307

Fig. 5-31

308

Fig. 5-32

309

Fig. 5-33

310

Fig. 5-34

314

Functions LSM 510

318

5-64 B 40-051 e 07/98

318

Measurement Concept

318

Fig. 5-36

319

Fig. 5-37

320

Fig. 5-39

327

Measured

329

Object Features

329

Volume Features

329

Zeiss LSM 510 META Manuale Utente (17 pagine)


Marchio: Zeiss | Categoria: Microscopi | Dimensione: 0.54 MB |

 

Indice

Power Up

1

Open the Software

1

Acquiring a confocal image

2

Choose a configuration

3

Z-sectioning

10

Time series

12

Ending your session

13

3-D stack display options

14




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