Zeiss LSM 510 META Specifiche Pagina 158

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OPERATION
Image Optimization LSM 510
4-100 B 40-051 e 07/98
For overlaying fluorescence and transmitted-light
images, click on the <Transmission> button on the
Beam Path and Channel Assignment panel.
The transmitted light PMT will be switched active.
Of course, all other transmitted light applications
like
phase contrast
differential interference contrast (DIC)
polarization contrast (Pol)
darkfield
can also be performed.
Image optimization must be performed as
described in the previous chapter.
For the generation of images in reflection, the main dichroic beam splitter must be a neutral-density
filter.
Standard equipment contains a neutral-density filter with a division ratio of 80 to 20 % (at 543 nm).
Fig. 4-108
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