Zeiss N HBO 103 Specifiche

Operating Manual Axio Observer Inverted microscope

INTRODUCTION Carl Zeiss Safety notes Axio Observer 10 B 46-0111 e 12/06 Axio Observer.A1 and .D1 (manual) are equipped with an integrated p

OPERATION Carl Zeiss TFT display touchscreen on the Axio Observer.Z1 Axio Observer 100 B 46-0111 e 12/06 (3) Buttons right An administrat

OPERATION Axio Observer TFT display touchscreen on the Axio Observer.Z1 Carl Zeiss B 46-0111 e 12/06 101 4.6.5.3 Extras The Settings/Extra

OPERATION Carl Zeiss TFT display touchscreen on the Axio Observer.Z1 Axio Observer 102 B 46-0111 e 12/06 (4) Ethernet This tab is used to

OPERATION Axio Observer TFT display touchscreen on the Axio Observer.Z1 Carl Zeiss B 46-0111 e 12/06 103 4.6.5.4 Info The Settings/Info pa

OPERATION Carl Zeiss Illumination and contrast techniques Axio Observer 104 B 46-0111 e 12/06 4.7 Illumination and contrast techniques 4.7

OPERATION Axio Observer Illumination and contrast techniques Carl Zeiss B 46-0111 e 12/06 105 1 Vertical adjustment knob for condenser

OPERATION Carl Zeiss Illumination and contrast techniques Axio Observer 106 B 46-0111 e 12/06 • Swivel the 3-position filter changer (4-65

OPERATION Axio Observer Illumination and contrast techniques Carl Zeiss B 46-0111 e 12/06 107 4.7.2 Setting up transmitted light phase cont

OPERATION Carl Zeiss Illumination and contrast techniques Axio Observer 108 B 46-0111 e 12/06 4.7.2.3 Setting up transmitted light phase c

OPERATION Axio Observer Illumination and contrast techniques Carl Zeiss B 46-0111 e 12/06 109 4.7.3 Setting up differential interference co

INTRODUCTION Axio Observer Safety notes Carl Zeiss B 46-0111 e 12/06 11 Dust and dirt may impair the performance of the instrument. The ins

OPERATION Carl Zeiss Illumination and contrast techniques Axio Observer 110 B 46-0111 e 12/06 1 Polarizer D (fixed, optionally rotatab

OPERATION Axio Observer Illumination and contrast techniques Carl Zeiss B 46-0111 e 12/06 111 (2) Transmitted light DIC with ±30° analyzer

OPERATION Carl Zeiss Illumination and contrast techniques Axio Observer 112 B 46-0111 e 12/06 4.7.4 Setting up transmitted light PlasDIC c

OPERATION Axio Observer Illumination and contrast techniques Carl Zeiss B 46-0111 e 12/06 113 4.7.5 Setting up transmitted light VAREL cont

OPERATION Carl Zeiss Illumination and contrast techniques Axio Observer 114 B 46-0111 e 12/06 − Shifting the VAREL illumination to outsid

OPERATION Axio Observer Illumination and contrast techniques Carl Zeiss B 46-0111 e 12/06 115 4.7.6 Setting up epifluorescence contrast 4.7

OPERATION Carl Zeiss Illumination and contrast techniques Axio Observer 116 B 46-0111 e 12/06 1 HBO 100 fluorescence illuminator 2

OPERATION Axio Observer Illumination and contrast techniques Carl Zeiss B 46-0111 e 12/06 117 • Select the FL reflector module with the req

OPERATION Carl Zeiss Documentation Axio Observer 118 B 46-0111 e 12/06 4.8 Documentation Axio Observer microscopes are fitted with up to f

OPERATION Axio Observer Documentation Carl Zeiss B 46-0111 e 12/06 119 Order no. / Viewed with / Intermediate Direction of Viewing angle,

INTRODUCTION Carl Zeiss Notes on warranty Axio Observer 12 B 46-0111 e 12/06 1.2 Notes on warranty The manufacturer guarantees that the ins

OPERATION Carl Zeiss Documentation Axio Observer 120 B 46-0111 e 12/06 Order no. / Viewed with / Intermediate Direction of Viewing angl

CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Axio Observer Care Carl Zeiss B 46-0111 e 12/06 121 5 CARE, MAINTENANCE, TROUBLESHOOTING AND

CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Carl Zeiss Maintenance Axio Observer 122 B 46-0111 e 12/06 5.2 Maintenance 5.2.1 Checki

CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Axio Observer Servicing Carl Zeiss B 46-0111 e 12/06 123 5.2.3 Replacing the fuses in the po

ANNEX Carl Zeiss List of abbreviations Axio Observer 124 B 46-0111 e 12/06 6 ANNEX 6.1 List of abbreviations a Free working distance

ANNEX Axio Observer List of abbreviations Carl Zeiss B 46-0111 e 12/06 125 M, mot. motorized Man Manual N. A. Numerical aperture Ph, P

ANNEX Carl Zeiss Index Axio Observer 126 B 46-0111 e 12/06 6.2 Index Page 3 3200 K ...

ANNEX Axio Observer Index Carl Zeiss B 46-0111 e 12/06 127 F Filter changer...

ANNEX Carl Zeiss Index Axio Observer 128 B 46-0111 e 12/06 Operation...

ANNEX Axio Observer Patent rights Carl Zeiss B 46-0111 e 12/06 129 6.3 Patent rights Instruments, instrument components or methods describe

INTRODUCTION Axio Observer Stand types (overview) Carl Zeiss B 46-0111 e 12/06 13 1.3 Stand types (overview)

ANNEX Carl Zeiss Axio Observer 130 B 46-0111 e 12/06

DESCRIPTION Carl Zeiss Designation and intended use Axio Observer 14 B 46-0111 e 12/06 2 DESCRIPTION 2.1 Designation and intended use Man

DESCRIPTION Axio Observer Description and main features Carl Zeiss B 46-0111 e 12/06 15 2.2 Description and main features The Axio Observer

DESCRIPTION Carl Zeiss Equipment and compatibility table Axio Observer 16 B 46-0111 e 12/06 2.3 Equipment and compatibility table Equipme

DESCRIPTION Axio Observer Equipment and compatibility table Carl Zeiss B 46-0111 e 12/06 17 Equipment Option A1 D1 Z1 6-pos. H DIC man. (

DESCRIPTION Carl Zeiss Equipment and compatibility table Axio Observer 18 B 46-0111 e 12/06 Equipment Option A1 D1 Z1 LD 0.35/0.55, manu

DESCRIPTION Axio Observer System overview Carl Zeiss B 46-0111 e 12/06 19 2.4 System overview Fig. 2-1 System overview (sheet 1)

Carl Zeiss Copyright / Trademarks Axio Observer 2 B 46-0111 e 12/06 Knowledge of this manual is required to operate the instrument. You sh

DESCRIPTION Carl Zeiss System overview Axio Observer 20 B 46-0111 e 12/06 Fig. 2-2 System overview (sheet 2)

DESCRIPTION Axio Observer System overview Carl Zeiss B 46-0111 e 12/06 21 1:1 Fig. 2-3 System overview (sheet 3)

DESCRIPTION Carl Zeiss System overview Axio Observer 22 B 46-0111 e 12/06 423646423646423646 Fig. 2-4 System overview (sheet 4)

DESCRIPTION Axio Observer Objectives Carl Zeiss B 46-0111 e 12/06 23 2.5 Objectives The objectives form the core of the microscope's op

DESCRIPTION Carl Zeiss Objectives Axio Observer 24 B 46-0111 e 12/06 The following objectives are available for the Axio Observer microscop

DESCRIPTION Axio Observer Eyepieces Carl Zeiss B 46-0111 e 12/06 25 2.6 Eyepieces The following eyepieces are recommended for the Axio Obser

DESCRIPTION Carl Zeiss Specimen stages Axio Observer 26 B 46-0111 e 12/06 2.8 Specimen stages Axio Observer microscopes can be fitted with

DESCRIPTION Axio Observer Technical specifications Carl Zeiss B 46-0111 e 12/06 27 2.10 Technical specifications Dimensions (width x depth

DESCRIPTION Carl Zeiss Technical specifications Axio Observer 28 B 46-0111 e 12/06 Power supply unit for HBO 100 Area of use...

SETTING UP THE INSTRUMENT Axio Observer Unpacking and installing the microscope Carl Zeiss B 46-0111 e 12/06 29 3 SETTING UP THE INSTRUMENT

Axio Observer Contents Carl Zeiss B 46-0111 e 12/06 3 CONTENTS Page 1 INTRODUCTION ...

SETTING UP THE INSTRUMENT Carl Zeiss Attaching the binocular (photo) tube Axio Observer 30 B 46-0111 e 12/06 3.2 Attaching the binocular (p

SETTING UP THE INSTRUMENT Axio Observer Attaching the binocular (photo) tube Carl Zeiss B 46-0111 e 12/06 31 3.2.2 Inserting the eyepiece r

SETTING UP THE INSTRUMENT Carl Zeiss Fitting the transmitted light illuminator Axio Observer 32 B 46-0111 e 12/06 3.3 Fitting the transmitt

SETTING UP THE INSTRUMENT Axio Observer Screwing in the objectives Carl Zeiss B 46-0111 e 12/06 33 3.4 Screwing in the objectives • Remove

SETTING UP THE INSTRUMENT Carl Zeiss Fitting the microscope stages Axio Observer 34 B 46-0111 e 12/06 3.5 Fitting the microscope stages 3.5

SETTING UP THE INSTRUMENT Axio Observer Fitting the microscope stages Carl Zeiss B 46-0111 e 12/06 35 3.5.2 Fitting a scanning stage • Sca

SETTING UP THE INSTRUMENT Carl Zeiss Fitting the microscope stages Axio Observer 36 B 46-0111 e 12/06 • After mounting the stage to the mic

SETTING UP THE INSTRUMENT Axio Observer Fitting the microscope stages Carl Zeiss B 46-0111 e 12/06 37 • Place the specimen stage (3-13/2) o

SETTING UP THE INSTRUMENT Carl Zeiss Attaching the condensers Axio Observer 38 B 46-0111 e 12/06 3.5.5 Fitting the gliding stage Z The glid

SETTING UP THE INSTRUMENT Axio Observer Attaching the condensers Carl Zeiss B 46-0111 e 12/06 39 3.6.2 Condensers from the Axio Imager rang

Carl Zeiss Contents Axio Observer 4 B 46-0111 e 12/06 3.9 Fitting the TFT display to the docking station...

SETTING UP THE INSTRUMENT Carl Zeiss Attaching the condensers Axio Observer 40 B 46-0111 e 12/06 3.6.3 Changing the DIC prism in the conden

SETTING UP THE INSTRUMENT Axio Observer Reflector turret Carl Zeiss B 46-0111 e 12/06 41 3.7 Reflector turret 3.7.1 Fitting the reflector

SETTING UP THE INSTRUMENT Carl Zeiss Reflector turret Axio Observer 42 B 46-0111 e 12/06 If the protecting glass for the reflector turret w

SETTING UP THE INSTRUMENT Axio Observer Reflector turret Carl Zeiss B 46-0111 e 12/06 43 If it is necessary to install filters without direc

SETTING UP THE INSTRUMENT Carl Zeiss Reflector turret Axio Observer 44 B 46-0111 e 12/06 • Tip up the excitation half of the module (3-24/1

SETTING UP THE INSTRUMENT Axio Observer Fitting the TFT display to the Z1 motorized stand Carl Zeiss B 46-0111 e 12/06 45 3.8 Fitting the T

SETTING UP THE INSTRUMENT Carl Zeiss Connecting the microscope to the mains power supply Axio Observer 46 B 46-0111 e 12/06 3.10 Connecting

SETTING UP THE INSTRUMENT Axio Observer Sockets on the rear of the Axio Observer Carl Zeiss B 46-0111 e 12/06 47 3.11 Sockets on the rear o

SETTING UP THE INSTRUMENT Carl Zeiss Sockets on the rear of the Axio Observer Axio Observer 48 B 46-0111 e 12/06 3.11.3 Z1 stand Key to Fig

SETTING UP THE INSTRUMENT Axio Observer Switching the microscope and the power supply for the HBO 100 on and off Carl Zeiss B 46-0111 e 12/06

Axio Observer Contents Carl Zeiss B 46-0111 e 12/06 5 4.7.5 Setting up transmitted light VAREL contrast ...

SETTING UP THE INSTRUMENT Carl Zeiss HAL 100 halogen illuminator Axio Observer 50 B 46-0111 e 12/06 3.13 HAL 100 halogen illuminator The HA

SETTING UP THE INSTRUMENT Axio Observer HAL 100 halogen illuminator Carl Zeiss B 46-0111 e 12/06 51 3.13.2 Adjusting the HAL 100 halogen il

SETTING UP THE INSTRUMENT Carl Zeiss HAL 100 halogen illuminator Axio Observer 52 B 46-0111 e 12/06 3.13.3 Fitting and removing the diffuse

SETTING UP THE INSTRUMENT Axio Observer HAL 100 halogen illuminator Carl Zeiss B 46-0111 e 12/06 53 3.13.4 Replacing the HAL 100 halogen bu

SETTING UP THE INSTRUMENT Carl Zeiss HBO 100 illuminator Axio Observer 54 B 46-0111 e 12/06 3.14 HBO 100 illuminator 3.14.1 Inserting the

SETTING UP THE INSTRUMENT Axio Observer HBO 100 illuminator Carl Zeiss B 46-0111 e 12/06 55 3.14.3 Adjusting the HBO 100 illuminator Two ve

SETTING UP THE INSTRUMENT Carl Zeiss Fitting the Aqua Stop II Axio Observer 56 B 46-0111 e 12/06 3.15 Fitting the Aqua Stop II The Aqua Sto

SETTING UP THE INSTRUMENT Axio Observer Attaching the mounting adaptor for Laserport to the stand Carl Zeiss B 46-0111 e 12/06 57 3.16 Atta

OPERATION Carl Zeiss Overview of components and controls Axio Observer 58 B 46-0111 e 12/06 4 OPERATION The Axio Observer microscope is av

OPERATION Axio Observer Overview of components and controls Carl Zeiss B 46-0111 e 12/06 59 Fig. 4-1 Axio Observer.A1 components and cont

Carl Zeiss Contents Axio Observer 6 B 46-0111 e 12/06 Fig. 3-26 Fitting the TFT display...

OPERATION Carl Zeiss Overview of components and controls Axio Observer 60 B 46-0111 e 12/06 4.1.2 Axio Observer.D1 (coded, semi-motorized)

OPERATION Axio Observer Overview of components and controls Carl Zeiss B 46-0111 e 12/06 61 Key to figure 4-2: 1 On / off button 2 Lef

OPERATION Carl Zeiss Overview of components and controls Axio Observer 62 B 46-0111 e 12/06 4.1.3 Axio Observer.Z1 (motorized) Fig. 4-3

OPERATION Axio Observer Overview of components and controls Carl Zeiss B 46-0111 e 12/06 63 Key to figure 4-3: 1 Standby button 2 Lef

OPERATION Carl Zeiss Description of components and controls Axio Observer 64 B 46-0111 e 12/06 4.2 Description of components and controls

OPERATION Axio Observer Description of components and controls Carl Zeiss B 46-0111 e 12/06 65 Nosepiece with objectives (4-1/5), (4-2/6), (

OPERATION Carl Zeiss Description of components and controls Axio Observer 66 B 46-0111 e 12/06 Manual condensers (4-1/8), (4-2/9) Depending

OPERATION Axio Observer Description of components and controls Carl Zeiss B 46-0111 e 12/06 67 Motorized condensers (4-3/8) − The turret i

OPERATION Carl Zeiss Description of components and controls Axio Observer 68 B 46-0111 e 12/06 Manual iris stop slider for reflected light

OPERATION Axio Observer Description of components and controls Carl Zeiss B 46-0111 e 12/06 69 Manual, coded or motorized 6-position reflect

Axio Observer Contents Carl Zeiss B 46-0111 e 12/06 7 Fig. 4-33 Page Microscope -> Control -> Light path...

OPERATION Carl Zeiss Description of components and controls Axio Observer 70 B 46-0111 e 12/06 Binocular tubes (4-1/19), (4-2/23), (4-3/22)

OPERATION Axio Observer Description of components and controls Carl Zeiss B 46-0111 e 12/06 71 Binocular section of binocular tubes (4-1/20)

OPERATION Carl Zeiss Description of components and controls Axio Observer 72 B 46-0111 e 12/06 Vertical limit stop for z focus drive (4-2/4

OPERATION Axio Observer Description of components and controls Carl Zeiss B 46-0111 e 12/06 73 Control ring, right (4-2/19), (4-3/17) − On

OPERATION Carl Zeiss Description of components and controls Axio Observer 74 B 46-0111 e 12/06 Docking station − For use with the Z1 stand

OPERATION Axio Observer Basic settings for the D1 stand Carl Zeiss B 46-0111 e 12/06 75 4.3 Basic settings for the D1 stand After switching

OPERATION Carl Zeiss Basic settings for the D1 stand Axio Observer 76 B 46-0111 e 12/06 During configuration, when a position (nosepiece an

OPERATION Axio Observer Light Manager on the Axio Observer.D1 and .Z1 Carl Zeiss B 46-0111 e 12/06 77 4.4 Light Manager on the Axio Observe

OPERATION Carl Zeiss Light Manager on the Axio Observer.D1 and .Z1 Axio Observer 78 B 46-0111 e 12/06 4.4.1 Light Manager mode: OFF Fig.

OPERATION Axio Observer Light Manager on the Axio Observer.D1 and .Z1 Carl Zeiss B 46-0111 e 12/06 79 4.4.3 Light Manager mode: SMART Fig.

INTRODUCTION Carl Zeiss Safety notes Axio Observer 8 B 46-0111 e 12/06 1 INTRODUCTION 1.1 Safety notes Axio Observer microscopes have been

OPERATION Carl Zeiss Light Manager on the Axio Observer.D1 and .Z1 Axio Observer 80 B 46-0111 e 12/06 4.4.5 Selecting and configuring Ligh

OPERATION Axio Observer Contrast Manager on the Axio Observer.Z1 Carl Zeiss B 46-0111 e 12/06 81 4.5 Contrast Manager on the Axio Observer.

OPERATION Carl Zeiss TFT display touchscreen on the Axio Observer.Z1 Axio Observer 82 B 46-0111 e 12/06 4.6 TFT display touchscreen on the

OPERATION Axio Observer TFT display touchscreen on the Axio Observer.Z1 Carl Zeiss B 46-0111 e 12/06 83 (2) Illumination The RL shutter but

OPERATION Carl Zeiss TFT display touchscreen on the Axio Observer.Z1 Axio Observer 84 B 46-0111 e 12/06 4.6.2 Menu overview The menu over

OPERATION Axio Observer TFT display touchscreen on the Axio Observer.Z1 Carl Zeiss B 46-0111 e 12/06 85 4.6.3 Home page After switching on,

OPERATION Carl Zeiss TFT display touchscreen on the Axio Observer.Z1 Axio Observer 86 B 46-0111 e 12/06 − TL Illumination / RL Illuminatio

OPERATION Axio Observer TFT display touchscreen on the Axio Observer.Z1 Carl Zeiss B 46-0111 e 12/06 87 (1) Objectives For objective positi

OPERATION Carl Zeiss TFT display touchscreen on the Axio Observer.Z1 Axio Observer 88 B 46-0111 e 12/06 (4) Light path The light path is d

OPERATION Axio Observer TFT display touchscreen on the Axio Observer.Z1 Carl Zeiss B 46-0111 e 12/06 89 (5) FluoArc The FluoArc tab allows

INTRODUCTION Axio Observer Safety notes Carl Zeiss B 46-0111 e 12/06 9 Axio Observer microscopes and original accessories are to be used for

OPERATION Carl Zeiss TFT display touchscreen on the Axio Observer.Z1 Axio Observer 90 B 46-0111 e 12/06 4.6.4.2 Automatic (1) Softkeys No

OPERATION Axio Observer TFT display touchscreen on the Axio Observer.Z1 Carl Zeiss B 46-0111 e 12/06 91 (a) Current position display / Set

OPERATION Carl Zeiss TFT display touchscreen on the Axio Observer.Z1 Axio Observer 92 B 46-0111 e 12/06 (2) Measure This tab is only acce

OPERATION Axio Observer TFT display touchscreen on the Axio Observer.Z1 Carl Zeiss B 46-0111 e 12/06 93 4.6.4.4 Incubation The Microscope/

OPERATION Carl Zeiss TFT display touchscreen on the Axio Observer.Z1 Axio Observer 94 B 46-0111 e 12/06 4.6.5 Settings Pressing Settings o

OPERATION Axio Observer TFT display touchscreen on the Axio Observer.Z1 Carl Zeiss B 46-0111 e 12/06 95 Pressing Empty pos. clears the cur

OPERATION Carl Zeiss TFT display touchscreen on the Axio Observer.Z1 Axio Observer 96 B 46-0111 e 12/06 (3) Focus This tab is used to ente

OPERATION Axio Observer TFT display touchscreen on the Axio Observer.Z1 Carl Zeiss B 46-0111 e 12/06 97 b) Parfocality The parfocality funct

OPERATION Carl Zeiss TFT display touchscreen on the Axio Observer.Z1 Axio Observer 98 B 46-0111 e 12/06 (6) Misc The Misc tab is used to c

OPERATION Axio Observer TFT display touchscreen on the Axio Observer.Z1 Carl Zeiss B 46-0111 e 12/06 99 4.6.5.2 User Pressing User on the