Zeiss LSM 510 META Specifiche Pagina 236
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OPERATION
Annex LSM 510
4-178 B 40-051 e 07/98
The shift is read off from the microscope stages. In the case of the manual Axioplan 2 stage, x can be
read directly from the scale adhered to the front of the stage. In the case of the manual Axiovert 100
stage, a scale is located on the right of the knob, where the 45 mm x shift relative to the zero position
of the microscope stage can be read off. The x value is positive for both stages if shift from the zero
position is made to the right and negative if the shift is made to the left.
On account of the inclined position of the stage tongue, the object is also shifted laterally during the fine
focusing motion. This lateral shift is negligibly small if, as recommended by us, specimen carriers with
thickness 1.0 mm are used exclusively. Otherwise, the marked lateral shift of the object during fine
focusing can result in image distortion. For the same reason, Petri dishes without fixation ring must be
used exclusively.
The nosepiece of the Axiovert stand is moved to the load position prior to switching off the LSM system
and the HRZ 200 is then moved to the lowest position to avoid damage of the objective or object by a
possible collision. The user must refocus after start-up of the system. Before an objective change in the
Axiovert or the Axioplan, the nosepiece and the microscope stage must be moved to the Load Position
by the user, and then back to the Work Position to prevent the objectives from hitting the HRZ
components. This is performed automatically if the objectives are changed menu-controlled via the
relevant buttons of the LSM program.
The HRZ 200 for the Axiovert 100 M (1013 186) or for the Axioplan 2
MOT
(1013 187) can be attached
to the following standard microscope stages:
mechanical stage 85 x 130 for Axiovert (45 13 39)
scanning stage DC 100 x 90 for Axiovert (45 17 40)
mechanical stages 75 x 50 for Axioplan (45 35 05, 45 35 02-99 04, 45 35 07)
scanning stage DC 4“ x 4“ for Axioplan (45 35 85-99 01)
In the case of the last configuration, the object plane is shifted upwards so that Köhler illumination and
classical transmitted-light microscopy will no longer be possible because the condenser cannot be moved
sufficiently close to the object.
The user will not have to deal with any other restrictions.
- Laser Scanning Microscope 1
- B 40-051 e 07/98 III 3
- IV B 40-051 e 07/98 4
- Scope of Equipment Supplied 6
- INTRODUCTION 10
- X B 40-051 e 07/98 10
- CONTENTS 11
- NOTES ON DEVICE SAFETY 12
- Contents LSM 510 12
- 1-2 B 40-051 e 07/98 12
- 1 NOTES ON DEVICE SAFETY 13
- Exit aperture 18
- B 40-051 e 07/98 1-9 19
- 1-10 B 40-051 e 07/98 20
- LGK 7774 21
- LGK 7628-1 21
- 1-12 B 40-051 e 07/98 22
- LSM 510 - SETUP REQUIREMENTS 24
- 2-2 B 40-051 e 07/98 24
- Fig. 2-1 25
- Fig. 2-2 25
- Fig. 2-3 26
- Fig. 2-4 27
- 2-12 B 40-051 e 07/98 34
- CHAPTER 3 INTRODUCTION TO 35
- LASER SCANNING MICROSCOPY 35
- 3-2 B 40-051 e 07/98 36
- Module VIS 40
- Scan ModuleMicroscope 40
- Module UV 40
- 3-12 B 40-051 e 07/98 46
- CHAPTER 4 OPERATION 59
- 4 OPERATION 61
- Fig. 4-1 62
- Fig. 4-2 63
- Fig. 4-3 63
- Fig. 4-4 64
- Fig. 4-5 65
- Fig. 4-6 66
- Fig. 4-7 66
- Fig. 4-8 67
- Fig. 4-9 67
- Fig. 4-10 68
- Fig. 4-11 69
- Fig. 4-12 70
- Fig. 4-13 71
- Fig. 4-14 72
- Fig. 4-15 73
- Fig. 4-16 74
- Fig. 4-17 75
- Fig. 4-18 76
- Fig. 4-19 77
- Fig. 4-20 78
- Fig. 4-21 79
- Fig. 4-22 80
- Fig. 4-23 82
- Fig. 4-24 82
- Fig. 4-25 83
- Fig. 4-26 83
- Fig. 4-27 83
- Fig. 4-28 84
- Fig. 4-29 84
- Fig. 4-30 85
- Fig. 4-31 86
- Fig. 4-32 88
- Fig. 4-33 89
- Fig. 4-34 90
- Fig. 4-35 90
- Fig. 4-36 91
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- Fig. 4-39 95
- Fig. 4-40 96
- Fig. 4-41 97
- Fig. 4-42 98
- Fig. 4-43 100
- Fig. 4-44 101
- Fig. 4-45 103
- OPERATION 104
- 4-46 B 40-51 e 07/98 104
- B 40-051 e 07/98 4-47 105
- Fig. 4-48 106
- Fig. 4-49 107
- Fig. 4-50 108
- Fig. 4-51 109
- Fig. 4-52 110
- Fig. 4-53 111
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- Fig. 4-55 113
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- Fig. 4-60 117
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- Fig. 4-63 120
- Fig. 4-64 121
- Fig. 4-65 122
- Fig. 4-66 123
- B 40-051 e 07/98 4-67 125
- Fig. 4-68 126
- Colored marker positions 126
- Fig. 4-69 127
- Fig. 4-70 128
- Fig. 4-71 129
- Fig. 4-72 129
- Fig. 4-73 130
- Fig. 4-74 130
- Fig. 4-75 131
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- Fig. 4-78 133
- Fig. 4-79 133
- Fig. 4-81 134
- Fig. 4-80 134
- Fig. 4-82 135
- Fig. 4-83 135
- Fig. 4-90 139
- Fig. 4-91 140
- LSM 510 Image Optimization 141
- B 40-051 e 07/98 4-83 141
- Fig. 4-93 142
- Fig. 4-94 143
- Fig. 4-95 144
- B 40-051 e 07/98 4-87 145
- Fig. 4-97 146
- Fig. 4-98 147
- Fig. 4-99 148
- Fig. 4-100 149
- Fig. 4-101 150
- Fig. 4-102 151
- Fig. 4-103 152
- Fig. 4-104 153
- / Ampl. Gain 154
- Fig. 4-105 155
- Fig. 4-106 156
- Fig. 4-107 157
- Fig. 4-108 158
- Fig. 4-109 159
- Fig. 4-110 160
- Fig. 4-111 161
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- 4-124 B 40-051 e 07/98 182
- Fig. 4-133 183
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- 4-134 B 40-051 e 07/98 192
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- B 40-051 e 07/98 4-137 195
- Fig. 4-146 196
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- Fig. 4-169 217
- LSM 510 Annex 219
- B 40-051 e 07/98 4-161 219
- Annex LSM 510 220
- 4-162 B 40-051 e 07/98 220
- B 40-051 e 07/98 4-163 221
- 4-164 B 40-051 e 07/98 222
- B 40-051 e 07/98 4-165 223
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- B 40-051 e 07/98 4-167 225
- 4-168 B 40-051 e 07/98 226
- B 40-051 e 07/98 4-169 227
- 4-170 B 40-051 e 07/98 228
- Fig. 4-170 Filter change 229
- Allen key 230
- B 40-051 e 07/98 4-173 231
- Fine focusing 233
- Fig. 4-173 234
- Fig. 4-174 237
- Fig. 4-175 240
- Fig. 4-176 246
- Fig. 4-177 247
- Fig. 4-178 249
- Fig. 4-179 250
- Fig. 4-180 252
- Fig. 4-181 253
- CHAPTER 5 3D FOR LSM 510 255
- 5-2 B 40-051 e 07/98 256
- Positive rotation directions 257
- Fig. 5-1 257
- Fig. 5-2 259
- Display window 260
- Fig. 5-4 265
- Fig. 5-5 267
- Fig. 5-6 268
- Fig. 5-7 270
- Fig. 5-9 274
- Fig. 5-10 275
- Fig. 5-11 276
- Fig. 5-12 278
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- Fig. 5-28 305
- Fig. 5-29 306
- Fig. 5-30 307
- Fig. 5-31 308
- Fig. 5-32 309
- Fig. 5-33 310
- Fig. 5-34 314
- Functions LSM 510 318
- 5-64 B 40-051 e 07/98 318
- Measurement Concept 318
- Fig. 5-36 319
- Fig. 5-37 320
- Fig. 5-39 327
- Measured 329
- Object Features 329
- Volume Features 329
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