Zeiss N HBO 103 Specifiche Scaricare il pdf (Pagina 106)

OPERATION

Carl Zeiss Illumination and contrast techniques Axio Observer

106 B 46-0111 e 12/06

• Swivel the 3-position filter changer (4-65/20) out of the optical path.

• Place a high-contrast specimen on the microscope stage (4-65/6).

• Adjust the eyepieces to the correct interpupillary distance by moving the binocular section (4-65/17) of

the binocular tube apart or together.

• Set the ametropia correction zero point using the adjustment ring (4-65/19) of the eyepieces (4-65/18)

with no eyepiece reticle: set to the white point,

with eyepiece reticle: set to the red point,

• To correct for ametropia, bring the selected detail of the specimen into optimum focus using the

relevant eyepiece adjustment ring.

• Use the coarse / fine focus drive (4-65/12) to bring the selected detail of the specimen into sharp

focus. If no light is visible in the eyepieces, check that light is being emitted from the housing of the

halogen illuminator. If no light is being emitted, switch on the halogen illuminator by pressing the TL

button (

4-65/13).

• Set the light intensity to a comfortable brightness using the illumination control (4-65/14).

• Close the luminous-field stop (4-65/21) until it

becomes visible in the field of view (not

necessarily in focus -

4-66/A).

• Bring the edge of the luminous-field stop into

focus (

4-66/B) by adjusting the height of the

condenser (

4-65/1).

• Center the luminous-field stop using the

centering screws (

4-65/2 - 4-66/C) and open

until the edge of the stop just disappears from

the field of view (

4-66/D).

• To set the aperture stop, remove one eyepiece from the eyepiece tube and adjust the aperture stop

(

4-65/5) to approximately 2/3 the diameter of the exit pupil of the eyepiece (4-66/E).

The settings required for optimum contrast depend on the specimen.

• Reinsert the eyepiece and where necessary refocus on the specimen using the fine focusing drive.

• Adjust the light intensity using the rocker switch.

The field size and objective aperture change each time the objective is changed, so that for

optimal results the luminous-field stop and aperture stop should be readjusted whenever the

objective is changed.

Fig. 4-66 Setting the stops for transmitted

light bright field KÖHLER

illumination

1 2 ... 101 102 103 104 105 106 107 108 109 110 111 ... 129 130

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Zeiss N HBO 103 Specifiche Pagina 106

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